Plasmid

Part:BBa_K4096003:Design

Designed by: Ke ZHU   Group: iGEM21_Shanghai_Metro_HS   (2021-10-12)


pET-25b-PKC-001


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 246
    Illegal XbaI site found at 380
    Illegal PstI site found at 4403
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 246
    Illegal NheI site found at 197
    Illegal PstI site found at 4403
    Illegal NotI site found at 219
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 246
    Illegal BglII site found at 446
    Illegal BamHI site found at 252
    Illegal XhoI site found at 212
    Illegal XhoI site found at 5572
    Illegal XhoI site found at 5644
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 246
    Illegal XbaI site found at 380
    Illegal PstI site found at 4403
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 246
    Illegal XbaI site found at 380
    Illegal PstI site found at 4403
    Illegal NgoMIV site found at 285
    Illegal NgoMIV site found at 478
    Illegal NgoMIV site found at 2066
    Illegal NgoMIV site found at 2226
    Illegal NgoMIV site found at 5406
    Illegal NgoMIV site found at 5680
    Illegal NgoMIV site found at 5878
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4229
    Illegal SapI site found at 3146


Design Notes

No


Source

No

References